Multiplex real-time PCR assay to detect illegal trade of CITES-listed shark species

Citation
Cardeñosa D, Quinlan J, Shea KH, Chapman DD (2018) Multiplex real-time PCR assay to detect illegal trade of CITES-listed shark species. Scientific Reports 8:. doi: 10.1038/s41598-018-34663-6
Abstract

The Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) is a multilateral environmental agreement to ensure that the international trade of threatened species is either prohibited (Appendix I listed species) or being conducted legally, sustainably, and transparently (Appendix II listed species). Twelve threatened shark species exploited for their fins, meat, and other products have been listed under CITES Appendix II. Sharks are often traded in high volumes, some of their products are visually indistinguishable, and most importing/exporting nations have limited capacity to detect illicit trade and enforce the regulations. High volume shipments often must be screened after only a short period of detainment (e.g., a maximum of 24 hours), which together with costs and capacity issues have limited the use of DNA approaches to identify illicit trade. Here, we present a reliable, field-based, fast (